Chlorogenic acid (1), scutellarin (2), syringin (3), 6S, 9R-roseoside (4) and beta-sitosterol-3-O-beta-D-glucopyranoside (5) were isolated from the aerial parts of Centaurea cadmea Boiss. (Asteraceae). Structure elucidation of the compounds were performed by using spectroscopic methods (1-D and 2-D NMR and LC-MS-MS). To the best of our knowledge, compounds 1, 2, 3 and 4 have been isolated for the first time from this endemic species. Compound 4 is new for the genus Centaurea.