Folic acid-modified clay: targeted surface design for cell culture applications

BONGARTZ R., AG D., SELECI M., WALTER J., YALÇINKAYA E. E. , Demirkol D. , ...Daha Fazla

JOURNAL OF MATERIALS CHEMISTRY B, cilt.1, ss.522-528, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 1 Konu: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1039/c2tb00328g
  • Sayfa Sayıları: ss.522-528


Here we report the preparation, characterization and application of folic acid modified Montmorillonite clay (FA-Mont) as a cell culture material. Clays exhibit unique properties such as good mechanical and chemical stabilities, high surface area, low toxicity and easy combination with functional organic groups. FA was used as a modifier to facilitate adhesion of folate positive cells on the clay surface. FA-Mont was characterized using FT-IR, XRD, zeta potential measurements as well as thermogravimetric analyses. Finally, the usage potential of FA-Mont as a receptor mediated cell adhesion material was successfully proved by using folate receptor (FR) rich HeLa and FR poor A-549 cells. The cell adhesion studies were monitored and imaged by fluorescence and scanning electron microscopy techniques.