Folic acid-modified clay: targeted surface design for cell culture applications


BONGARTZ R., AG D., SELECI M., WALTER J., YALÇINKAYA E. E. , Demirkol D. , ...More

JOURNAL OF MATERIALS CHEMISTRY B, vol.1, no.4, pp.522-528, 2013 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 1 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1039/c2tb00328g
  • Title of Journal : JOURNAL OF MATERIALS CHEMISTRY B
  • Page Numbers: pp.522-528

Abstract

Here we report the preparation, characterization and application of folic acid modified Montmorillonite clay (FA-Mont) as a cell culture material. Clays exhibit unique properties such as good mechanical and chemical stabilities, high surface area, low toxicity and easy combination with functional organic groups. FA was used as a modifier to facilitate adhesion of folate positive cells on the clay surface. FA-Mont was characterized using FT-IR, XRD, zeta potential measurements as well as thermogravimetric analyses. Finally, the usage potential of FA-Mont as a receptor mediated cell adhesion material was successfully proved by using folate receptor (FR) rich HeLa and FR poor A-549 cells. The cell adhesion studies were monitored and imaged by fluorescence and scanning electron microscopy techniques.