Dipeptidyl peptidase IV production by solid state fermentation using alternative fungal sources


Ongen G. , SARGIN S. , ÜSTÜN O., Kutlu C., Yucel M.

TURKISH JOURNAL OF BIOLOGY, cilt.36, ss.665-671, 2012 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 36 Konu: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3906/biy-1201-26
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Sayfa Sayısı: ss.665-671

Özet

The present work was carried out for the production of dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) using Aspergillus, Penicillium, and Rhizopus strains under solid state fermentation conditions. Response surface methodology was applied for the optimization of the selected operational variables (corn flour, initial moisture content, and cultivation time) for DPP IV activity as the response. The optimal parameters of DPP IV activity for the independent variables, namely the amount of corn flour (% w/w), initial moisture content (% w/w), and cultivation time (days), were evaluated to be 2.44%, 60.85%, and 4.69 days, respectively, using Aspergillus awamori T116. The response for these results was also shown to be in very close agreement with the experimental data.