Akademik Veri Yönetim Sistemi
2 INTERNATIONAL EURASIAN CONFERENCE ON SCIENCE, ENGINEERING AND TECHNOLOGY (EurasianSciEnTech 2020), Gaziantep, Türkiye, 7 - 09 Ekim 2020, ss.374-379
Abstract
Increase in carbon dioxide and other
greenhouse gases in the atmosphere are the main cause of global warming. The
biggest impact of global warming is climate change. Syngas is a mixture of CO,
CO2, N2, H2 and CH4 gases that are
obtained through gasification and pyrolysis of biomass. Syngas fermentation results
in production of ethanol and acetic acid from CO by a set of biochemical
reactions known Wood-Ljungdahl pathway under anaerobic conditions. In this context,
conversion of synthetic gas to bioethanol, which is an alternative biofuel to
fossil fuels, is considered to be a promising approach in mitigating the
negative effects of global warming by reducing greenhouse gas emissions.
The main driving argument of this study is
to offer an economically sustainable basal media which is rich in trace elements, since the current commercial
trace elements mixtures requires the purchase of highly expensive chemical components to prepare the
required basal environment. Therefore, it is crucial to optimise the necessary
recipe for trace element in order to reduce the potential production costs. For
this purpose, Plackett Burman statistical method was used to select the most
effective trace elements (Ni, Mg, Ca, Mn, Co, Cu, B, W, Zn, Fe and Mo) in batch
experiments using Clostridium ljungdahlii at 37oC.
As a result of 11 experimental sets which were determined by Plackett Burman
method, an optimized trace element recipe was formulized. It was observed that the use of trace elements
increased ethanol production approximately 3.5 times, the effect of Ni was negligible,
and the model created was statistically significant (p = 0.0228 <0.05). Optimum trace
element combination was determined to be as follows; 0.02 g/L Na2WO4
x 2H2O, 0.75 g/L FeSO4 x 7H2O, 0.02 g/L NiCl2
x 6H2O, 0.01 g/L MgSO4 x 7H2O, 0.79 g/L MnSO4
x H2O, 0.20 g/L CoCl2 x 6H2O, 0.01 g/L CuCl2
x 2H2O, 0.01 g/L H3BO3.
Keywords: Trace elements, Syngas fermentation, Bioethanol, Clostridium
Ljungdahlii