There are a quite few studies which determine optimum splicing settings and examine thoroughly the effect of these settings on the properties of the spliced yarn zone based on yarn properties. There is a lack of studies especially about the effect of fiber properties on the spliced zone. In this study, in order to obtain maximum tenacity, and elongation after splicing of the yarns which have different fiber properties, yarn counts and yarn twists, optimum splicing settings were tried to be determined. The effects of splicing parameters, fiber and yarn properties on the tenacity and elongation of the spliced yams were investigated in detail. For this reason, yarns from 8 different blends which were supplied from different yarn spinning mills, having three different counts (Ne 20, Ne 30 ve Ne 40) and three different twist coefficients (alpha e 3,8; alpha e 4,2 ve alpha e 4,6) were spun. Fiber properties in the roving form which were measured via using HVI fiber testers were evaluated separately. Response surface methodology was used to analyze spliced yarn tenacity and elongation as dependent variable by choosing fiber properties together with the machine settings such as opening air, splicing air and splicing time, yarn twist and yarn count as independent variables. As a result of the study, equations which predict the tenacity and elongation of the spliced yarns. are statistically important and have high coefficient of multiple determination (R(2)) were obtained.