Immobilization of urease on activated methoxypolyethyleneglycol-5000


Hamarat Ş. , Uslan A.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, vol.24, no.3, pp.273-283, 1996 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 3
  • Publication Date: 1996
  • Doi Number: 10.3109/10731199609117439
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
  • Page Numbers: pp.273-283

Abstract

Urease (E.C 3.5.1.5) was covalently immobilized on activated methoxypolyethyleneglycol-5000 which is linear, uncharged, soluble in water and nonimmunogenic. mPEG is bound to the epsilon-NH2 groups of Lysin in urease. Previously different molar ratios of urease -Lys / activated-mPEG were searched for immobilization. Storage stabilities, molecular weights and the values of blocked amino groups were determined for each immobilized urease and the best conditions was found 1:3 urease-lys / activated mPEG. Furthermore physical characterization, kinetic constants (K-m, V-max), heat and temperature stabilites were also determined.