Tissue culture of Echinacea angustifolia allows for selection and rapid multiplication of genetically superior clones. In this study, temporary immersion liquid bioreactor (RITA(R) system), synthetic seed technology and semi-solid culture conditions were evaluated for shoot multiplication efficiency. After 25 days of culture, the highest multiplication rate was observed in explants cultured in temporary immersion liquid system. Previous reports on micropropagation of E. angustifolia have had low success rate in rooting. Shoots were rooted under autotrophic conditions on sugar-free semi-solid media supplemented with 2.5 muM IBA and using synthetic seed technology by shoot encapsulation in calcium alginate gel cultured on MS medium supplemented with 2.6 muM NAA. Rooting response was noticed in three weeks with a success rate of 83%. Arbuscular mycorrhiza infection helped the acclimatization survival rate of rooted plantlets.