Tomato plants (cv. Primadonna F-1), non-grafted, self-grafted, or grafted onto the commercial rootstocks He-man' and Maxifort', were grown in recirculating nutrient solution. The uptake concentrations (UCs), i.e. mean nutrient-to-water uptake ratios of N, P, K, Ca, Mg, Fe, Mn, Zn, Cu, and B, were estimated based either on depletion from the nutrient solution or on accumulation in the plant biomass. Grafting onto both commercial rootstocks increased the total plant biomass. Hetero-grafting also increased the leaf N, Ca, and Cu concentrations but decreased those of Mg and Fe in comparison with self- and non-grafted plants. The mean UCs of N, Ca, and Cu were higher in plants grafted onto both commercial rootstocks in comparison with self- and non-grafted plants. However, hetero-grafting also raised the UCs of P, Fe, Mn, and B, because of an increased deposition of these nutrients to the roots in comparison with self-rooted plants. The method used to estimate the UCs, i.e. nutrient removal from the recirculating nutrient solution vs. nutrient recovery from plant biomass per volume unit of transpired water, resulted in similar values for N, Ca, Zn, and Cu, but had a significant impact on those of P, K, Mg, Fe, Mn, and B.