The harmonized INFOGEST in vitro digestion method: From knowledge to action


Egger L., Menard O., Delgado-Andrade C., Alvito P., Assuncao R., Balance S., ...Daha Fazla

FOOD RESEARCH INTERNATIONAL, cilt.88, ss.217-225, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 88
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.foodres.2015.12.006
  • Dergi Adı: FOOD RESEARCH INTERNATIONAL
  • Sayfa Sayıları: ss.217-225

Özet

Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas beta-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase.