Optimization of Arthrospira sp. (Spirulina) Growth: From Laboratory Scale to Pilot Outdoor Culture

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ISS2019 4th International Science Symposium ”New Horizons in Science, Kiev, Ukraine, 4 - 06 September 2019, vol.2, no.20192, pp.170-177

  • Publication Type: Conference Paper / Full Text
  • Volume: 2
  • City: Kiev
  • Country: Ukraine
  • Page Numbers: pp.170-177


In this study, the culture of Arthrospira sp. isolated from Çamaltı saltern ecosystem was studied. Arthrospira sp. biomass productivity was investigated through medium choice. Type MZ modified Zarrouk’s medium was selected as it gave higher final dry weights and longer sustained growth than Zarrouk’s culture media. Then, in order to reduce Spirulina species production cost, modified Zarrouk’s medium was rationalized by testing different compond. It was found that modified Zarrouk’s medium could be diluted up to twice times without impacting the growth rates in a 20-21 days batch cultivation. Higher dry weights were even observed after 21 days of batch cultivation (1.26±3g/L for 50%- MZ in comparison to 0.90±3 g/L for Zarrouk’s medium). Photosynthetically Photon Flux Density (PPFD) from 1800 lux. to 2000 lux. At 2000 lux. light intensity, photoinhibition was not observed and the specific growth rate was maximum (0.13±1/day). Finally, a 21-day cultivation experiment was conducted in a 2000mL culture erlenmayer flaks giving a maximum daily volume productivity of 70±2mg/L/day. A techno-economic analysis according to Turkish economicall terms gave production cost two to 20 times higher for culture medium MZ (from 20 to 80€/kg) than for open ponds (from 6 to 12-15€/kg) depending on Spirulina productivity. Keywords: Arthrospira, Biomass, Culture, Çamaltı Saltern, İzmir