Purification of alpha-galactosidase by affinity precipitation with alginate


Celem E. B. , Onal S.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.38, ss.348-357, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 38 Konu: 4
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/10826060802325436
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Sayfa Sayıları: ss.348-357

Özet

Affinity precipitation is a technique which is known for over 20 years, but has recently received more attention due to the development of new materials for its implementation. It is a relatively simple, convenient, and reproducible technique that results in high target molecule recovery at high specificity. We describe, here, an efficient and rapid purification procedure for Vicia faba -galactosidase (EC 3.2.1.22) by using affinity precipitation with alginate. The enzyme was purified with 43% activity yield and 40-fold purification. SDS-PAGE of the purified enzyme showed a single band and a subunit weight of 44kDa. The properties of the enzyme were also searched. The results showed that the general properties of the enzyme offer potential for use of this -galactosidase in several production processes.