A total of 111 Sepiola intermedia specimens were collected from the Aegean Sea at seasonally between 2007 and 2008. The maturity stages of both sexes and the gonadosomatic index values were estimated. It was observed that the ovulation pattern of S. intetmedia is asynchronous and spawning occurs continuously. Potential fecundity ranges for females and males were determined to be between 148-406 eggs (mean 271) and 54-383 spermatophores (mean 197), respectively. The females with mantle lengths ML between 14-22 mm obtained 68 to 161 spermantangia (mean 96) ranging from 0.3-0.9 mm in length throughout the season inside their bursa copulatrix. The mantle lengths of mature females varied from 15 to 22 mm ML, those of mature males between 15 and 23 mm ML. This study provides new records on the reproductive biology of S. intetmedia.