The Effects of Sodium Laureth Sulphate On Zebrafish Liver Organotypic Culture


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GÖKÇE B., İŞİSAĞ ÜÇÜNCÜ S.

2nd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2019), 28 - 29 Haziran 2019, ss.1536-1541

  • Sayfa Sayıları: ss.1536-1541

Özet

Anionic surfactant sodium laureth sulphate (SLES) is a foaming agent and used in the main component of most

commercial products. Because of its low cost and effective utilization, it is used in many cosmetic products for

their cleaning and emulsifying properties. However, in case of common use, surfactants are widely distributed in

the environment especially aquatic habitats on a global scale. Risk analyses are the main process for any kind of

xenobiotics and in vitro toxicological methods are becoming widely used for assessment in these evaluations. A

major promise of in vitro systems is to obtain mechanism derived information that is considered pivotal for

adequate risk assessment. In this study, biochemical and histological effects of SLES were evaluated in zebrafish

3D organotypic liver culture system. Four different concentrations (0.1, 0.5, 1.0 and 1.5 μg/ml) exposed for 24

and 96 hours to organotypic culture of liver samples. Catalase (CAT), glutathione-S-transferase (GST) enzyme

activities and lipid peroxidation levels were analysed for biochemical parameters. Exposed tissue samples were

evaluated with the routine histological procedure and compared with non-exposed samples for histopathological

assessment. Results were showed that enzyme activities and lipid peroxidation levels were increased depending

on increasing concentration both 24 and 96 hours exposure group. Vacuolizations, nuclear abnormalities, necrotic

areas were observed in histopathological observations. All of these findings were indicated that SLES have

potentially had liver toxicity on zebrafish organotypic liver culture system.