Chemical constituents and biological activities of Dianthus elegans var. elegans


MUTLU K., Sarikahya N. , NALBANTSOY A. , Kirmizigul S.

NATURAL PRODUCT RESEARCH, cilt.32, ss.1245-1253, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 32 Konu: 11
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/14786419.2017.1333988
  • Dergi Adı: NATURAL PRODUCT RESEARCH
  • Sayfa Sayıları: ss.1245-1253

Özet

Chemical investigation of the aerial parts of Dianthus elegans var. elegans afforded two previously undescribed saponins, named dianosides M-N (1-2), together with four oleanane-type triterpenoid glycosides (3-6). Their structures were elucidated as 3-O--L-arabinofuranosyl-16-hydroxyolean-12-ene-23, 28-dioic acid (1) and 3-O--L-arabinofuranosyl-(13)--D-glucopyranosyl 16-hydroxyolean-12-ene-23-oic acid, 28-O--D-glucopyranosyl-(16)--D-glycosyl ester (2) by chemical and extensive spectroscopic methods including IR, 1D, 2D NMR and HRESIMS. Both of the saponins were evaluated for their cytotoxicities against HEK-293, A-549 and HeLa human cancer cells using the MTT method. All compounds showed no substantial cytotoxic activity against tested cell lines. However, dianosides M-N and the n-butanol fraction exhibited considerable haemolysis in human erythrocyte cells. The immunomodulatory properties of dianosides M-N were also evaluated in activated whole blood cells by PMA plus ionomycin. Dianosides M-N increased IL-1 concentration significantly whereas the n-butanol fraction slightly augmented IL-1 secretion. All compounds did not change IL-2 and IFN- levels considerably.