Imaging of bacterial infection with Tc-99m-labeled HBD-1


Lambrecht F. , Yilmaz O., ÜNAK P. , SEYITOGLU B., DURKAN K. , YOLCULAR S., ...Daha Fazla

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.277, ss.555-559, 2008 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 277 Konu: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s10967-007-7135-x
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Sayfa Sayıları: ss.555-559

Özet

The aim of this study was to evaluate Tc-99m labeled human beta-defensin-1 (HBD-1) for discrimination between bacterial infection and sterile inflammation. For this purpose, HBD-1 was radiolabeled with 99mTc and its in vivo distribution was evaluated in inflamed rats with Staphylococcus aureus (S. aureus) and sterile inflamed rats with turpentine oil. After injection into inflamed and sterile inflamed rats, Tc-99m-HBD-1 was rapidly removed from the circulation via the kidneys. Binding of Tc-99m-HBD-1 to inflamed muscle (T/NT 20 at 120 min) was two times higher than binding to sterile inflamed muscle (T/NT = 10 at 120 min) of rats. It was demonstrated that Tc-99m-HBD-1 can be used to detect S. aureus inflammation in rats. However, the radiolabeled antimicrobial peptide showed only poor uptake in sterile inflammation with turpentine oil in rats. As a result, Tc-99m-HBD-1 can be useful for detection of bacterial inflammation.