Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus


Yarali E., ERDEM GÜRSAN K. A.

MICROMACHINES, vol.12, no.7, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 7
  • Publication Date: 2021
  • Doi Number: 10.3390/mi12070753
  • Title of Journal : MICROMACHINES
  • Keywords: cobalt phthalocyanine, ionic liquid, voltammetric biosensor, Hepatitis B virus, pencil graphite electrode, differential pulse voltammetry, SINGLE-USE SENSOR, CARBON NANOTUBES, ELECTROCHEMICAL DETECTION, IMPEDANCE SPECTROSCOPY, GRAPHITE-ELECTRODES, BIOSENSOR, FILM, NANOPARTICLES, SURFACE

Abstract

In this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning electron microscopy (SEM), and the electrochemical behavior of electrodes were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The voltammetric detection of hybridization was investigated by evaluating the guanine oxidation signal, measured by differential pulse voltammetry (DPV) technique. The implementation of our biosensor to serum samples was also examined using fetal bovine serum (FBS). The detection limit was established as 0.19 mu g/mL in phosphate buffer solution (PBS) (pH 7.40) and 2.48 mu g/mL in FBS medium. The selectivity of our assay regarding HBV DNA hybridization in FBS medium was tested in the presence of other DNA sequences. With this aim, the hybridization of DNA probe with non-complementary (NC) or mismatched DNA sequence (MM), or in the presence of mixture samples containing DNA target NC (1:1) or DNA target MM (1:1), was studied based on the changes in guanine signal.