SUBSTRATE-SPECIFICITY AND THE USE OF CHITOSAN-ENTRAPPED RABBIT HEPATIC-MICROSOMAL UDP-GLUCURONYL TRANSFERASE FOR DETOXIFICATION


ZIHNIOGLU F. , TELEFONCU A.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, cilt.23, ss.533-543, 1995 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23 Konu: 4
  • Basım Tarihi: 1995
  • Doi Numarası: 10.3109/10731199509117968
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
  • Sayfa Sayıları: ss.533-543

Özet

Rabbit hepatic microsomal UDP-Glucuronyl transferase (EC.2.4.1.17) was immobilized in chitosan, which is an ionotropic gelatation agent, and the resulting preparation was used as a biocatalyst for the glucuronidation of toxic substances and drugs such as; l-naphthol, phenol, 4-nitrophenol, bilirubin, testesterone, estrone, lamotrigine, imipramine and chlorpromazine. For studying the drug metabolism in vitro and obtaining artificial liver support (detoxification) chitosan-entrapped UDPGT towards l-naphthol was tested by using two model reactor system. The conversion curves of l-naphthol to its glucuronide indicated that the column reactor system was found to be better and seems suitable for detoxification with a high yield of glucuronide formation.