An organic solvents tolerant and thermotolerant lipase from Pseudomonas fluorescens P21


ÇADIRCI B. H. , YAŞA İ.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, cilt.64, ss.155-161, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 64
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.molcatb.2009.09.009
  • Dergi Adı: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
  • Sayfa Sayıları: ss.155-161

Özet

In this study, 38 organic solvents tolerant bacteria have been isolated from 21 different soil and water environments and the bacterium called P21 has been isolated among them due to its ability of surviving in 30% toluene. which is highly toxic to microorganisms and its capability of high lipase production (3.5 U/I). The lipase production medium has been optimized and the medium containing olive oil as an inducer and peptone as a nitrogen source has been selected as the best ingredients for the highest lipase production conditions (15 U/I). After this optimization, initial lipase activity has increased to 638.3%.