In the present research, the responses of the tissue culture of five explant types (first leaf, leaf, epicotyl, hypocotyl and apical shoot tip) of alfalfa (Medicago sativa L.) cv. Kayseri in two different basic media (MS and PC-L2) supplemented with different concentrations of 6-benzyladenine (BA; 1, 3, and 5 mg/L) combined with NAA (1-naphthalene acetic acid; 1 mg/L) were evaluated. The highest number of shoots per explant was obtained from shoot tip explants. Comparing BA concentration levels, the inclusion of 5mg/L BA in the media was more effective on number of shoots per explant than the other levels. Shoot height formed from epicotyl and shoot tip explants was higher than the other explant types. Generally, all of explant types in both media reached the highest height at the 1 mg/L BA concentration level.