CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments


Donmez A. , Tombuloglu M. , Gulbahar O. , Arik B., Cagirgan S., Vural F. , ...Daha Fazla

TRANSFUSION AND APHERESIS SCIENCE, cilt.49, ss.307-312, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 49 Konu: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.transci.2013.08.006
  • Dergi Adı: TRANSFUSION AND APHERESIS SCIENCE
  • Sayfa Sayıları: ss.307-312

Özet

No detailed information currently exists about the immune phenotypic profiles of peripheral blood stem cells (PBSCs) obtained by different mobilization regimens. The effects of these profiles on the outcome of transplantation are largely unknown. In this prospective study, the surface immune phenotypic features (CD11a, CD18, CD31, CD38, CD44, CD62e, CD62L, CD90, CD117, CD135 and CD184 expression) of sorted PBSCs that had been mobilized by growth factor with (group I and group II) or without (group III) disease-specific chemotherapies were investigated. The immune phenotypic features on mobilized PBSCs in groups I, II and III were not significantly different. The CD31 (platelet endothelial cell adhesion molecule-1) positivity ratio on PBSCs inversely correlated with both the duration of neutrophil (r = -0.32, p = 0.03) and platelet (r = -0.36, p = 0.02) engraftment. No relationship was found between the engraftment (neutrophil and platelet) durations and CD184 (chemokine receptor CXC motif receptor 4 [CXCR4]) expression on PBSCs. We demonstrated that the surface immune phenotypic profiles on PBSCs obtained by several mobilization regimens were not different. To our knowledge, this is the first study to demonstrate that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Contrary to our expectations, CD184 (CXCR4) expression on PBSCs has no effect on neutrophil or platelet engraftment. Considered together, our results suggest that additional surface antigens (such as CD31) may be more effective in the homing process. (C) 2013 Elsevier Ltd. All rights reserved.