Electrochemical detection of interaction between capsaicin and nucleic acids in comparison to agarose gel electrophoresis


YILMAZ N., EKSİN E., KARAÇİÇEK B., ERAÇ Y. , ERDEM A.

ANALYTICAL BIOCHEMISTRY, cilt.535, ss.56-62, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 535
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.ab.2017.07.023
  • Dergi Adı: ANALYTICAL BIOCHEMISTRY
  • Sayfa Sayıları: ss.56-62

Özet

In this study, the biomolecular interaction occurred between nucleic acids and Capsaicin (CPS), the active compound in chilli peppers, which has been reported to have anti-carcinogenic properties, was investigated for the first time herein using disposable electrochemical biosensor. It is aimed to perform the surface-confined interaction between CPS and different types of nucleic acids and under this aim, the experimental conditions were optimized; such as, the concentration of CPS and DNA, DNA immobilization time and interaction time etc. The detection limit of DNA was estimated based on guanine oxidation signal in the linear concentration range of DNA from 1 to 5 mu g/mL, and it was found to be 0.62 mu g/mL. The effect of time-dependent manner from 1 min to 30 min on the interaction of CPS with nucleic acids was explored upon to the changes at guanine signal coming from double stranded DNA and cDNA as well as PCR samples. The interaction of CPS with double stranded DNA was also determined by agarose gel electrophoresis. (C) 2017 Elsevier Inc. All rights reserved.