Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum. vulgare subsp spontaneum) populations from west Turkey


Tanyolac B.

GENETIC RESOURCES AND CROP EVOLUTION, vol.50, no.6, pp.611-614, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 6
  • Publication Date: 2003
  • Doi Number: 10.1023/a:1024412814757
  • Title of Journal : GENETIC RESOURCES AND CROP EVOLUTION
  • Page Numbers: pp.611-614

Abstract

Inter-Simple Sequence Repeat (ISSR) and Randomly Amplified Polymorphic DNA ( RAPD) markers were used to analyze genetic distance among H. vulgare subsp. spontaneum populations from west Turkey. Fifty-five RAPD and 10 ISSR primers were used to detect variation among sample. A total of 55 polymorphic loci were found using 65 primers. Two distinct cluster groups were clearly established among populations. The minimum variation was detected between Pinarbasi and Bornova ( GD = 0.192) populations and the maximum was found between Icmeler and Aydin populations ( GD = 0.926). As two dominant markers, RAPD and ISSRs are effective and promising marker systems for detecting genetic variation.