The applicability of madder by using printing technique over various natural fibers was investigated in the study. The effect of different factors, i.e. dye and urea concentration, type of fixation, fixation temperature and time, effect of mordant type and mordanting methods were studied. Printed fabrics were evaluated by means of color values and fastness properties. The K/S increases rapidly as the concentration of the natural dye powder in the printing paste increases from 15 to 45 g/kg printing paste. The effect of mordant on color was also studied. The results show that the highest K/S value was obtained by using mordant. Application of madder with the printing technique is expected to bring a different and an ecological alternative to new designs.