Determination of vitamin K-1 content in olive oil, chard and human plasma by RP-HPLC method with UV-Vis detection

Otles S. , ÇAĞINDI O.

FOOD CHEMISTRY, vol.100, no.3, pp.1220-1222, 2007 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 100 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.foodchem.2005.12.003
  • Title of Journal : FOOD CHEMISTRY
  • Page Numbers: pp.1220-1222


The contents of phylloquinone (Vitamin K-1) were investigated in olive oil, chard and human plasma based on a reversed phase high performance liquid chromatographic method with acetonitrile/dichloromethane/methanol (60:20:20, v/v/v) as eluent in a C-18 mu-Bondapak column (10 mu m, 300 x 3.9 mm) at 20 degrees C. Detection was by UV-Vis detector at 248 nm. In olive oil, the mean content of phylloquinone ranged from 12.7 to 18.9 mu g/100 g, in chard ranged from 65.5 to 77.5 mu g/100 g. In human plasma, phylloquinone content varied between 0.22 and 0.56 ng/ml. (c) 2005 Elsevier Ltd. All rights reserved.