Determination of vitamin K-1 content in olive oil, chard and human plasma by RP-HPLC method with UV-Vis detection


Otles S. , ÇAĞINDI O.

FOOD CHEMISTRY, cilt.100, ss.1220-1222, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 100 Konu: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.foodchem.2005.12.003
  • Dergi Adı: FOOD CHEMISTRY
  • Sayfa Sayıları: ss.1220-1222

Özet

The contents of phylloquinone (Vitamin K-1) were investigated in olive oil, chard and human plasma based on a reversed phase high performance liquid chromatographic method with acetonitrile/dichloromethane/methanol (60:20:20, v/v/v) as eluent in a C-18 mu-Bondapak column (10 mu m, 300 x 3.9 mm) at 20 degrees C. Detection was by UV-Vis detector at 248 nm. In olive oil, the mean content of phylloquinone ranged from 12.7 to 18.9 mu g/100 g, in chard ranged from 65.5 to 77.5 mu g/100 g. In human plasma, phylloquinone content varied between 0.22 and 0.56 ng/ml. (c) 2005 Elsevier Ltd. All rights reserved.