Incorporation of EDTA for the elimination of metal inhibitory effects in an amperometric biosensor based on mushroom tissue polyphenol oxidase


Erdem A. , Ozkan D. , MERIC B., KERMAN K., OZSOZ M.

TURKISH JOURNAL OF CHEMISTRY, cilt.25, ss.231-239, 2001 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 25 Konu: 2
  • Basım Tarihi: 2001
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.231-239

Özet

Enzyme based biosensors are highly selective devices which rely on the specific binding of the target analyte (the substrate) to the active-site regions of the enzyme. The enzymatic reaction between mushroom tissue polyphenol oxidase and its substrate phenol is coupled with the use of potassium ferrocyanide (K4Fe(CN)(6)) as the mediator in ammonia buffer solution (pH 8.80). The response of devices is often affected by the presence of inhibitors, which combine with the free enzyme in a manner that prevents substrate binding.