Incorporation of EDTA for the elimination of metal inhibitory effects in an amperometric biosensor based on mushroom tissue polyphenol oxidase


Erdem A. , Ozkan D. , MERIC B., KERMAN K., OZSOZ M.

TURKISH JOURNAL OF CHEMISTRY, vol.25, no.2, pp.231-239, 2001 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 2
  • Publication Date: 2001
  • Title of Journal : TURKISH JOURNAL OF CHEMISTRY
  • Page Numbers: pp.231-239

Abstract

Enzyme based biosensors are highly selective devices which rely on the specific binding of the target analyte (the substrate) to the active-site regions of the enzyme. The enzymatic reaction between mushroom tissue polyphenol oxidase and its substrate phenol is coupled with the use of potassium ferrocyanide (K4Fe(CN)(6)) as the mediator in ammonia buffer solution (pH 8.80). The response of devices is often affected by the presence of inhibitors, which combine with the free enzyme in a manner that prevents substrate binding.