In this study, cadmium (Cd) and chromium (Cr) phytoremediation responses of different tobacco varieties were compared and genetic differences between these varieties were analyzed. Two pot experiments were established with four oriental tobacco varieties, Akhisar, Basma, Dibek and Saribaglar, which were grown for 50 days in media containing 0, 10, 50, 100 and 150 mg/kg of Cd and Cr. Plants were departed as root, stem and leaf, and analyzed for their Cd and Cr amounts. In this regard, Cd and Cr distribution and uptake rates in different plant parts of the varieties were determined. Bioconcentration factor (BCF) values, which are considered to be important criteria for phytoremediation, were also calculated.