By virtue of their small size, nanomaterials have wide applications. Silver nanoparticles were synthesized by a reduction method in this study. Obtained solution was subjected to Ag+ ions and glucose determinations, pH, turbidity, and particle size estimations. Prepared different types of polyurethane composites were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope-energy-dispersive spectroscopy, atomic force microscopy, and scratch testing. The antibacterial activity against Escherichia coli and Staphylococcus aureus has been studied applying an in vitro test. The bactericidal activity for these bacteria cells has been estimated by zone of inhibition on the nutrient agar plates.