Objective: This study was design to evaluate the relationship between cigarette, alcohol, substance experience and use disorders and gender, faculty, class, living environment, substance use of families among university students. Method: The research was cross-sectional area study and sample were selected stratified sampling method was designated according to faculty and class level. Application was carried out in classroom. Demographic data form, Fagerstrom Nicotine Test for Dependence and Alcohol Use Disorders Identification Test were used. Frequency tests, Pearson chi-square, Mann Whitney U and Kruskal Wallis and Spearman correlation tests were used in statistical evaluation. Results: 1522 students were attended the survey. Cigarette, alcohol, substance experience and tobacco, alcohol disorders among men found higher than in women. Cannabis (12.5%), ecstasy (MDMA) (2%), cocaine (0.6%) and heroin (0.1%) were mostly used illegal drugs. There was a relationship between cigarette, alcohol, substance experience, alcohol use disorders and class, faculty, living environment and familial substance use. There was no association found between any factors except gender and tobacco use disorder. Conclusion: Among university students, being a male, studying in upper classes and having familial drug use can lead to increase of experience and addicted to substances of a person. Staying with own family or at a dorm, reading in faculties representing the health sciences can be considered as protective factors. The findings of tobacco use disorder have showed that using or being addicted to cigarette is not a distinctive feature among young smokers.