Electrochemical Monitoring of Nucleic Acid Hybridization by Single-Use Graphene Oxide-Based Sensor


Muti M., SHARMA S., Erdem A. , Papakonstantinou P.

ELECTROANALYSIS, vol.23, no.1, pp.272-279, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201000425
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.272-279
  • Keywords: Graphene oxide, DNA hybridization, Electrochemistry, Guanine, Disposable graphite electrode, Sensors, WALLED CARBON NANOTUBES, DIRECT DNA HYBRIDIZATION, MODIFIED ELECTRODES, GRAPHITE-ELECTRODES, BIOSENSOR SYSTEMS, RECOGNITION, DIAGNOSTICS, COMPOSITES

Abstract

In this paper, the application of a Graphene oxide (GRPox) integrated on single-use pencil graphite electrode (PGE) for the enhanced monitoring of nucleic acids and for the sensitive and selective detection of the label free DNA hybridization related to Hepatitis B virus (HBV) sequences is described. Electrochemical behaviors of GRPox modified PGEs were firstly investigated using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined voltammetrically in the case of hybridization between amino linked probe and its complementary (target), or, noncomplementary (NC), or target/mismatch (MM) mixtures (1:1).