Evaluation of a multi-parameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise

Orhan H. , Van Holland B., KRAB B., MOEKEN J., Vermeulen N., HOLLANDER P., ...More

FREE RADICAL RESEARCH, vol.38, no.12, pp.1269-1279, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 12
  • Publication Date: 2004
  • Doi Number: 10.1080/10715760400013763
  • Title of Journal : FREE RADICAL RESEARCH
  • Page Numbers: pp.1269-1279


The objective of the present. study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6 +/- 0.7) exercised 60 min at 70% of maximal O-2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise. As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD).