The present study was carried out to investigate the effect of hydroponic and substrate types of soilless culture on fruit quality of the tomato variety "Gokce 191 F-1" grown under greenhouse conditions during 1996-1997. Flowing water culture (F.W.C.), a type of water culture, was provided using 6 meter long PVC pipes having a diameter of 16 cm. Pots filled with peat-perlite in a ratio of 3:1 and pumice were used as types of substrate culture. Quality characteristics like average fruit weight, average fruit diameter, 1st, 2nd, 3rd grade fruit yield, total soluble solids (TSS), titratable acidity (TA), pH, and EC of the juice were determined. Fruits were also examined for colour, vitamin C content, fruit flesh firmness and dry matter content.