ANALYSIS OF VITAMIN-A IN EGGS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY


OTLES S. , HISIL Y.

NAHRUNG-FOOD, cilt.35, ss.391-394, 1991 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 35 Konu: 4
  • Basım Tarihi: 1991
  • Doi Numarası: 10.1002/food.19910350421
  • Dergi Adı: NAHRUNG-FOOD
  • Sayfa Sayıları: ss.391-394

Özet

A high speed liquid chromatographic method was described for the rapid determination of vitamin A in eggs. After saponification and extraction, the sample was separated on a reversed-phase column (C18) with acetonitrile - methylene-chloride - methanol as the mobile phase. Quantitative determination was accomplished by monitoring the ultraviolet absorbance of retinol at 280 nm. Recovery of vitamin A was 97.1 %.