Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line


İLEM ÖZDEMİR D. , KARAVANA S. Y. , ŞENYİĞİT Z., ÇALIŞKAN Ç. , EKİNCİ M. , AŞIKOĞLU M. , ...Daha Fazla

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.310, ss.515-522, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 310 Konu: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s10967-016-4805-6
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Sayfa Sayıları: ss.515-522

Özet

In the present study incorporation differences between the developed formulations for treatment for bladder cancer was investigated by radioactive cell culture studies. Developed bioadhesive microspheres (MS) and gemcitabinehydrochloride loaded MS (GHCl-MS) were radiolabeled. After observing the optimum labeling conditions, Tc-99m-MS and Tc-99m-GHCl-MS were loaded to the chitosan gel (CG) and poloxamer gel. The in vitro cell incorporation affinity of newly developed formulations to bladder papilloma (RT4) and the carcinoma (T24) cell lines was investigated. Cell culture studies results indicate that the use of prolonged release bioadhesive CG formulation was highly improved the targeting of the GHCl to the cancer cells.