Poly(HEMA-co-NBMI) Monolithic Cryogel Columns for IgG Adsorption


Uygun M., SENAY R. H. , AVCIBAŞI N. , AKGÖL S.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.172, ss.1574-1584, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 172 Konu: 3
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s12010-013-0624-1
  • Dergi Adı: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.1574-1584

Özet

Supermacroporous poly(2-hydroxyethyl methacrylate-co-1,5-naphthalene bismaleimide) [poly(HEMA-co-NBMI)] monolithic cryogel column was prepared by free radical cryo-copolymerization of HEMA with NBMI as a hydrophobic functional comonomer and N,N'-methylene-bisacrylamide as cross-linker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix which has inter-connected pores of 10-100 mu m size. Poly(HEMA-co-NBMI) cryogel was characterized by swelling studies, FTIR and scanning electron microscopy. The equilibrium swelling degree of the poly(HEMA-co-NBMI) cryogel was 10.5 g of H2O/g dry cryogel. Poly(HEMA-co-NBMI) cryogel was used in the adsorption/desorption of IgG from aqueous solutions. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was 98.20 mg/g polymer at pH 7.0. The nonspecific adsorption of IgG onto plain poly(HEMA) cryogel was very low (2.79 g/g polymer). It was observed that IgG could be repeatedly adsorbed and desorbed with the poly(HEMA-co-NBMI) cryogel without significant loss of adsorption capacity.