Use of Bacteria as Fluorescent Penetrant for Penetrant Testing (PT)

Biber Müftüler F. Z. , Karatay Kutman M., Harmanşah C. , Kozguş Güldü Ö.

Journal Of Nondestructive Evaluation, cilt.39, ss.1-15, 2020 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 39 Konu: 15
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10921-020-0653-y
  • Dergi Adı: Journal Of Nondestructive Evaluation
  • Sayfa Sayıları: ss.1-15


Penetrant testing (PT) is a Nondestructive testing (NDT) method used for identifying and revealing surface cracks and

defects in components. Using bacteria-based suspension as a fluorescent penetrant leads a new approach to PT with the

advantages of using eco-friendly chemicals, fewer steps for test process and achieving low cost. The aim of the current study

is to synthesize a new fluorescein isothiocyanate (FITC) conjugated Escherichia coli (E. coli) bacteria (FITC-E. coli) as

fluorescent penetrant for detection of cracks on test materials. For this purpose, we tested performance of proposed bacteria

suspension by means of its detection sensitivity using standardize test panels. Crack centers (6.35, 3.97 and 2.38 mm) and

defects (0.2 × 0.4, 0.3 × 0.4 and 0.4 × 0.4 mm) were successfully detected via FITC-E. coli. Experimental results showed that

the FITC-E. coli is efficient for detecting the cracks and defects. Research into development of bacteria-based fluorescent

penetrant may bring new opportunities for NDT applications in the future.