Specific determination of hydrogen peroxide with a catalase biosensor based on mercury thin film electrodes


Ertas F. N. , TIMUR S. , Akyilmaz E. , DINCKAYA E.

TURKISH JOURNAL OF CHEMISTRY, cilt.24, ss.95-99, 2000 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 24 Konu: 1
  • Basım Tarihi: 2000
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.95-99

Özet

A biosensor for specific determination of hydrogen peroxide was developed using catalase (EC 1.11.1.6) in combination with a mercury film electrode. Catalase was immobilized with gelatine by means of glutaraldehyde on the electrode surface. The biosensor response was monitored by following the reduction peak of dissolved oxygen at around -0.24 V. The peak current changes upon addition of hydrogen peroxide and gives a linear response in a concentration range of 1-50 x 10(-6) M within a response time of 3 minutes.