A study on polymerase chain reaction-based HLA DQ proportional to locus in Elazig, Turkey


Tokdemir M., Dulger H., Akin H. , Doymaz M.

AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY, cilt.21, ss.184-188, 2000 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 21 Konu: 2
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1097/00000433-200006000-00018
  • Dergi Adı: AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY
  • Sayfa Sayıları: ss.184-188

Özet

The polymerase chain reaction (PCR) was used for genetic characterization of 45 samples taken from the city of Elazig in Turkey. The polymorphism at the human leukocyte antigen DQ proportional to locus was detected. Allele and genotype frequencies were determined for unrelated individuals at this locus. Laboratory analyses were done by PCR amplification of DNA. Hybridization to allele specific oligonucleotide probes was performed using a reversed dot-blot typing method. The collected genotype and allele frequencies have been tested, and a comparison was made with other population surveys of this locus. Allele frequencies ranged from 3.3% (allele 1.3) to 36.7% (allele 4), with a discrimination power of 0.92, No deviation was seen from Hardy-Weinberg equilibrium in the findings.