Isolation and Immobilization of His-Tagged Alcohol Dehydrogenase on Magnetic Nanoparticles in One Step: Application as Biosensor Platform


GULDU O. K. , ECE S., EVRAN S. , MEDİNE E. İ. , Demirkol D. , ÜNAK P. , et al.

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, cilt.51, ss.699-705, 2014 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 51 Konu: 9
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/10601325.2014.936236
  • Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
  • Sayfa Sayısı: ss.699-705

Özet

His-tagged Alcohol dehydrogenase was produced as a recombinant protein in E. coli. Afterwards, isolation and immobilization of the enzyme was carried in one-step via copper modified magnetic nanoparticles (MNPs) by the effect of interactions between Cu and histidine. The resulting enzyme bound MNPs was then attached to the surface of carbon paste electrode by the magnetic force and used as an electrochemical biosensor for the alcohol sensing applications.