Evaluation of conjunctival swab sampling in the diagnosis of canine leishmaniasis: A two-year follow-up study in Cukurova Plain, Turkey


KARAKUŞ M., TÖZ S. , Ertabaklar H., Pasa S., Atasoy A., Arserim S. K. , ...More

VETERINARY PARASITOLOGY, vol.214, pp.295-302, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 214
  • Publication Date: 2015
  • Doi Number: 10.1016/j.vetpar.2015.09.009
  • Title of Journal : VETERINARY PARASITOLOGY
  • Page Numbers: pp.295-302
  • Keywords: Canine leishmaniasis, Leishmania infantum, Conjunctival swab, Nested-PCR, IFAT, CUTANEOUS LEISHMANIASIS, VISCERAL LEISHMANIASIS, ENZYMATIC POLYMORPHISM, EXPANDING ZOONOSIS, INFANTUM INFECTION, SOUTHERN ANATOLIA, CLINICAL-SAMPLES, DONOVANI, STRAINS, REGION

Abstract

The diagnosis of canine leishmaniasis (CanL) in symptomatic and asymptomatic dogs is a very important and problematic public health issue in Turkey. A longitudinal study was carried out on dogs in selected villages in the Cukurova Plain in Turkey, from July 2011 to June 2013, where cutaneous (CL) and visceral (VL) leishmaniasis is endemic. The study aimed to determine the prevalence of CanL and to evaluate the early diagnostic performance of the non-invasive conjunctival swab nested PCR (CS n-PCR) test in comparison with the Indirect Fluorescent Antibody Test (IFAT). The consecutive blood and CS samples from a representative number of dogs (80-100 dogs/each survey) were collected in a cohort of 6 villages located in the area. Clinical symptoms, demographic and physical features about each dog were noted and lymph node aspiration samples were obtained from selected dogs with lymphadenopathy.