Antitumor Efficacy of Quercetin and Resveratrol in PANC-1 Pancreatic Cancer Cell Line


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Ekmen B., Gürdal M., Karadadaş E., Özpolat B., Ercan G.

2nd International Cancer and Ion Channels Congress , İzmir, Türkiye, 22 - 24 Eylül 2019, ss.1-4

  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayısı: ss.1-4

Özet

Background: By most anti-cancer researchs, the phenolic compounds, Quercetin and Resveratrol are offered as adjuvant therapies to potentiate and/or to reduce the side effects of the chemotherapeutics. Cancer studies have shown that Quercetin and Resveratrol may also reduce Eukaryotic elongation factor 2 kinase (eEF2K) expression.

Objective: To evaluate the effects of Quercetin and Resveratrol in PANC-1 Pancreatic Cancer (PaCa) cell line in vitro.

Materials and Methods: The IC50 values of the polyphenols were determined by cytotoxicity tests. To evaluate their effects on PANC-1 cells, cellular migration, wound-healing and colony forming assays were used. Real-Time PCR was used to determine the changes in gene expression levels of eEF2K.

Results: The IC50 values of Quercetin (7.75 µM), Resveratrol (48.75 µM) in PANC-1 cells were determined at 72th hours. Polyphenol application at the given doses decreased colony forming ability and migration of PANC-1 cells. eEF2K gene expression was decreased with Quercetin but increased with Resveratrol treatment.

Conclusions: Based on these results, Quercetin has a stronger effect with lower IC50 doses. Therefore, it might be recommended alone or in combination with chemotherapeutics.