2nd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2019), Ankara, Türkiye, 28 - 29 Haziran 2019, ss.826
Recently, there is lots of biotechnological applications which needs nanoparticles such as biomaterials, biosensors, nanomedicine, diagnostic kits, enzyme immobilization and purification (Cao et. al., 2012). The main purposes of enzyme immobilization by magnetic nanoparticles are provide protection from advers environmental conditions, combine different types of enzyme for simplify processes that require complex operations, increase reaction yield by large surface area and ensure re-use of enzyme for subsequent applications (Arsalan and Younus, 2018). This particles can be used also for purification of enzymes by nonspecific or specific binding. Because they easily seperate by magnetic field, they provide saving of time without complicated operation such as centrifugation and filtration. Fe, Co and Ni and their oxidized forms are the most commonly used magnetic nanoparticles. Magnetic nanoparticles has some advantages like relatively cheap production methods, large surface area, simply seperation by magnetic field and low toxicity (Ma et. al., 2019). This particles could be commonly synthesed by physical, chemical and biological methods. Each methods can be divided into specific techniques according to equipments, chemical components and physical/chemical/biological environmental conditions (Fatima and Kim, 2017). It’s not enough the succesfully synthesis of the nanoparticles for directly using. The surface of this nanoparticles should be modified for stabilization and functionalization. By this way, nanoparticles can be used for long time and more specifically interract with enzymes (Lu et. al.,2007). We aimed to introduce synthesis, modification and enzmatic applications of magnetic nanoparticles by this presentation.