A new support material for IgG adsorption: Syntrichia papillosissima (Copp.) Loeske


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Demir M. E. , Uygun D. A. , Erdag A., AKGÖL S.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.45, ss.1363-1368, 2017 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 45 Konu: 7
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/21691401.2017.1296848
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayısı: ss.1363-1368

Özet

In this presented work, Syntrichia papillosissima (Copp.) Loeske (S. papillosissima) was used as a natural phytosorbent for IgG purification. These moss species were collected for the natural habitat and prepared for IgG adsorption studies by cleaning, drying, and grinding to uniform size. Syntrichia papillosissima samples were characterized by using FTIR and SEM studies. Functional groups of S. papillosissima were identified by FTIR analysis, while surface characteristics were determined by SEM studies. A batch system was used for the adsorption of IgG onto S. papillosissima surface and physical conditions of the IgG adsorption medium were investigated by modifying the pH, IgG concentration and temperature. Maximum IgG adsorption onto S. papillosissima was found to be 68.01 mg/g moss by using pH 5.0 buffer system. Adsorption kinetic isotherms were also studied and it was found that, Langmuir adsorption model was appropriate for this adsorption study. Reusability profile of S. papillosissima was also investigated and IgG adsorption capacity did not decrease significantly after 5 reuse studies. Results indicated that S. papillosissima species have the capacity to be used as biosorbent for IgG purification, with its low cost, natural and biodegradable structure.