Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system


TİMUR S. , Odaci D. , DINCER A., ZİHNİOĞLU F. , TELEFONCU A.

TALANTA, cilt.74, ss.1492-1497, 2008 (SCI İndekslerine Giren Dergi)

Özet

Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O-2 consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 mu M nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD). (C) 2007 Elsevier B.V. All rights reserved.