The importance of Stevia rebaudiana Bertoni is increasing due to including steviol glycosides that are zero-calorie sweeteners. This study was conducted in order to determine and compare the morphological and cytological characteristics of Stevia rebaundiana plants obtained from different locations in Turkey (Bayindir, Kusadasi, Burhaniye, Gursu and Bafra) under field conditions in Izmir. The experiment was carried out using a randomized complete block design replicated three times in the Faculty of Agriculture of Ege University. According to the results obtained, the tallest plants measured were those from the Burhaniye and Gursu genotypes. The maximum number of branches was found in the Burhaniye genotype. While the maximum number of leaves was observed on plants from the Gursu genotype, the maximum fresh herb and dry herb yields were obtained from the Burhaniye genotype. The maximum dry leaf yield and the maximum chlorophyll content (SPAD value) were also seen in the Burhaniye genotype. As a result, it was determined that the highest herb yields were obtained from stevia plants of the Burhaniye genotype. This study emphasizes the importance of genotype selection by considering the morphological and cytological characteristics of stevia plants for future polyploidy breeding studies in Mediterranean climate conditions.