Electrochemical Detection of Solution Phase Hybridization Related to Single Nucleotide Mutation by Carbon Nanofibers Enriched Electrodes


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Erdem A. , Eksin E.

MATERIALS, vol.12, no.20, 2019 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 20
  • Publication Date: 2019
  • Doi Number: 10.3390/ma12203377
  • Title of Journal : MATERIALS
  • Keywords: carbon nanofibers, solution-phase nucleic acid hybridization, zip nucleic acids, screen printed electrodes, electrochemical impedance spectroscopy, FACTOR-V-LEIDEN, IMPEDIMETRIC DETECTION, CHAIN-REACTION, IMPEDANCE SPECTROSCOPY, DNA HYBRIDIZATION, ANTICANCER DRUG, NUCLEIC-ACIDS, BIOSENSOR, SENSOR, ARRAY

Abstract

In the present study, a sensitive and selective impedimetric detection of solution-phase nucleic acid hybridization related to Factor V Leiden (FV Leiden) mutation was performed by carbon nanofibers (CNF) modified screen printed electrodes (SPE). The microscopic and electrochemical characterization of CNF-SPEs was explored in comparison to the unmodified electrodes. Since the FV Leiden mutation is a widespread inherited risk factor predisposing to venous thromboembolism, this study herein aimed to perform the impedimetric detection of FV Leiden mutation by a zip nucleic acid (ZNA) probe-based assay in combination with CNF-SPEs. The selectivity of the assay was then examined against the mutation-free DNA sequences as well as the synthetic PCR samples.