Material of Pycnogonida was collected by scuba diving and snorkelling in the eastern Mediterranean Sea from the Aegean Sea to northern Cyprus in 1995-2002; some additional samples were taken in 2006 (mainly in Gencelli Cove, Turkey). This yielded the first record of Ammothella appendiculata for the region. A. longioculata and Callipallene spectrum are newly recorded for Turkey. A hitherto undescribed and tiny species of Anoplodactylus was found. The insufficiently known adult stage of Ammothella uniunguiculata is figured by SEM photograph.