Determination of Cut-Off Titers and Agreement Between Immunofluorescence and Immunoblotting Methods for Detecting Antinuclear Antibodies in Children


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AKSU G. , GULEZ N., AZARSIZ E. , KARACA N. , KÜTÜKÇÜLER N.

JOURNAL OF CLINICAL LABORATORY ANALYSIS, vol.24, no.4, pp.230-236, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 4
  • Publication Date: 2010
  • Doi Number: 10.1002/jcla.20391
  • Title of Journal : JOURNAL OF CLINICAL LABORATORY ANALYSIS
  • Page Numbers: pp.230-236
  • Keywords: ANA, immunoflourescence, immunoblotting, cut-off titer, children, CONNECTIVE-TISSUE DISEASES, DIFFERENT TEST SYSTEMS, AUTOANTIBODY DETECTION, NUCLEAR ANTIGENS, HEP-2 CELLS, ANA, TESTS, DIAGNOSIS, DSDNA, ENA

Abstract

Detection of antinuclear antibodies (ANA) is a diagnostic adjunct in patients with suspected autoimmune connective tissue diseases, and various detection methods are in use. The aim of this study was to analyze the agreement between the ANA immunoflourescence (IF) and immunoblotting (IB) methods and determine cut-off for children subjects in a laboratory setting. We evaluated 729 serum samples that were analyzed by both ANA IF and IB. The results were evaluated by chi(2) test and, for agreement, kappa index was used. Frequencies determined for both 1:40-1:100 cut-off titers of ANA IF in relation to IB testing supported the idea that 1:100 starting dilution should be recommended in children subjects for ANA IF method and antigen specific immunoblot testing was needed, especially for some of the ANA IF negative samples. Agreement between the two methods, especially with homogenous, granular, and nucleolar ANA IF patterns, was statistically significant. J. Clin. Lab. Anal. 24:230-236, 2010. (C) 2010 Wiley-Liss, Inc.