In this study, the responses of Asian tobacco varieties to chromium stress were investigated. To determine the responses arising from genotypic differences, Basma and Dubek varities were used. Basma was suitable for removing Cr from soil and was more tolerant than Dubek to chromium stress. A significant variation in stomatal characters, except for stomatal width, was observed in both varieties. In parallel with the increasing doses of Cr, stomata density significantly increased in both genotypes. Also, it was observed that stomatal length decreased with increases in Cr dosage. The increase in Cr concentration leads to decrease in SPAD value. It was observed that the SPAD value of Basma was more than Dubek in control plants. The correlation of stomatal parameters with each other and SPAD values were also calculated. A negative correlation was found between the SPAD value and stoma density in both varieties. Also, a positive correlation was observed between SPAD value and stomatal length. There was a significant negative correlation between stoma density and stomatal length.