One-step conjugation of glycylglycine with [F-18]FDG and a pilot PET imaging study


Senısık A. M. , Ichedef C. , Kilcar A. Y. , Ucar E., Ari K., Goksoy D., et al.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.316, ss.457-463, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 316
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10967-018-5772-x
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Sayfa Sayısı: ss.457-463

Özet

This study describes a single step conjugation of Glycylglycine (GlyGly) which is a small peptide, with [F-18]FDG via oxime formation. Amiooxy-functionalization of GlyGly (AO-GlyGly) was accomplished through the reaction of Boc-aminooxy succinimide ester. Conjugation reaction was performed at 100 A degrees C for 30 min in a vial containing AO-GlyGly and [F-18]FDG solution. The radiolabeled product ([F-18]FDG-GlyGly) was obtained with 98.65 +/- 0.35% yield without any purification step which makes this method more attractive for F-18 radiolabeling. The present study is concluded with an in vivo pilot animal PET study to assess biodistribution and kinetics of chemoselectively [F-18]FDG tagged GlyGly in vivo.