A biosensor based on graphite epoxy composite electrode for aspartame and ethanol detection


ANIK U., Odaci D. , Timur S. , MERKOCI A., ALEGRET S., BESUN N., ...More

ANALYTICA CHIMICA ACTA, vol.570, no.2, pp.165-169, 2006 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 570 Issue: 2
  • Publication Date: 2006
  • Doi Number: 10.1016/j.aca.2006.04.010
  • Title of Journal : ANALYTICA CHIMICA ACTA
  • Page Numbers: pp.165-169
  • Keywords: bienzyme biosensor, aspartame, ethanol, graphite epoxy composite electrode (GECE), INJECTION SPECTROPHOTOMETRIC DETERMINATION, SOLID-PHASE REACTOR, POLYMER BIOCOMPOSITES, ALCOHOL OXIDASE, RIGID CARBON, PHOSPHATE

Abstract

A gelatin membrane with carboxyl esterase and alcohol oxidase was subsequently integrated onto the surface of a graphite epoxy composite electrode (GECE). The developed biosensors showed linearity in the range of 2.5-400 mu M for aspartame and 2.5-25 mu M for ethanol with response times of 170 and 70 s for each analyte, respectively. The resulting bienzyme biosensor was used for aspartame detection in diet coke samples and ethanol detection in beer and wine samples. From the obtained results, it can be concluded that the developed biosensor is a selective, practical and economic tool for aspartame and ethanol detection in real samples. (c) 2006 Elsevier B.V. All rights reserved.